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兔源多克隆抗大电导钙激活钾通道抗体 WB 大电导钙激活钾通道

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  • 询价
  • AB5228-200UL
  • 2026年01月01日
  • Western Blotting
  • Rabbit
  • Mouse;Rat;Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围

      Western Blotting

    • 宿主

      Rabbit

    • 抗原来源

      0

    • 适应物种

      Mouse;Rat;Human

    • 库存

      大量

    • 是否单克隆

      2

    • 规格

      200 μL

    Product Images

    Western blotting of non-differentiated PC12 cells lysate (30 μg)

    1. Anti-P2X2 antibody (1:200)

    2. Anti-P2X2 antibody, preincub...

    Species Reactivity Key Applications Host Format Antibody Type
    M, R, H WB Rabbit Affinity Purified Polyclonal Antibody
    Description:
    Anti-Potassium Channel MaxiK, calcium activated, aa1097-1196
    Alternate Names:
    • BKCa; Slo; KCNMA1
    • KCa1.1
    Specificity:
    Corresponds to the C-terminus of the longer form of BKCa channel α subunit and overlaps by ~40 residues with a shorter form and not with other known proteins tested so far.
    Key Applications:
    Western Blotting
    Application Notes:
    Western blot: 1:200 using ECL on rat brain membranes.
    Note: The addition of 0.1% Tween to the standard milk block is recommended.
    Immunohistochemistry on rat brain sections.
    Dilutions should be made using a carrier protein such as BSA (1-3%)
    Optimal working dilutions must be determined by the end user.
    Species Reactivity:
    • Mouse
    • Rat
    • Human
    Immunogen:
    A purified fusion protein (GST) and a C-terminal part (residues 1097-1196) of mouse KCa 1.1 channel (Accession Q08460-2).
    Epitope:
    C-terminal sequence SHSSHSSQ
    Control:
    CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For positive control, in Western blotting, use 20 ng protein per lane. For negative control, preincubate 3-5 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
    Presentation:
    Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg five minutes).
    Storage Conditions:
    Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
    Gene Symbol:
    • KCNMA1
    • OTTHUMP00000064161
    • OTTHUMP00000060198
    • KCNMA
    • K
    • OTTHUMP00000064158
    • MaxiK
    • VCAlpha)
    • SLO-ALPHA
    • MGC71881
    • OTTHUMP00000064157
    • SAKCA
    • OTTHUMP00000064156
    • OTTHUMP00000064164
    • Slo1
    • BKTM
    • OTTHUMP00000064165
    • hSlo
    • mSLO1
    • OTTHUMP00000064154
    • Slo-alpha
    • SLO
    • OTTHUMP00000064162
    • KCa1.1
    • OTTHUMP00000064155
    • OTTHUMP00000064160
    • DKFZp686K1437
    • OTTHUMP00000064159
    Antibody Category:
    Neuroscience
    Antibody Sub-Category:
    Ion Channels & Transporters
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Therapeutic Areas:
    Inflammation
    Format:
    Affinity Purified
    Host:
    Rabbit
    Concentration:
    60 μg/200 μL (after reconstitution)
    Antibody Type:
    Polyclonal Antibody

    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    A purified fusion protein (GST) and a C-terminal part (residues 1097-1196) of mouse KCa1.1 channel (Accession Q08460-2). 详细描述见链接:http://www.millipore.com/catalogue/item/AB5228-200UL

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    图标文献和实验
    相关实验
    • 免疫共沉淀(Co-IP)详细步骤教程

      或另一类单抗 兔多克隆抗体:正常兔IgG 在免疫共沉淀实验中要保证实验结果的真实性,应注意以下几点: (1) 确保共沉淀的蛋白是由所加入的抗体沉淀得到的,而并非外源非特异蛋白,单克隆抗体的使用有助于避免污染的发生; (2) 要确保抗体的特异性,即在不表达抗原的细胞溶解物中添加抗体后不会引起共沉淀; (3) 确定蛋白间的相互作用是发生在细胞中,而不是由于细胞的溶解才发生的,这需要进行蛋白质的定位来确定。

    • 细胞内细胞因子的流式细胞仪检测

      孵育10分钟。(剂量参照说明书)③ 加23mLPBS洗液,离心500g 5分钟,弃上清。5、细胞内染色① 加入荧光标记的细胞因子抗体,混匀,室温暗处孵育30分钟。② 加2、3mL洗液,离心500g 5分钟,弃上清,加入500LPBS上机或加入500 L 1% PFA固定后再上机七、注意事项1、标本处理避免使用络合钙的抗凝剂,如ACD与EDTA,因为它们会限制依赖性激活过程,推荐用肝素钠。此外LPS,一种常见的生物试剂污染物,是强细胞激活剂,可能会混淆试验结果。血样在8小时内分析,超过8小时

    • 细胞内细胞因子的流式细胞技术检测

      说明书) ③ 加23mLPBS洗液,离心500g 5分钟,弃上清。 5、细胞内染色 ① 加入荧光标记的细胞因子抗体,混匀,室温暗处孵育30分钟。 ② 加2、3mL洗液,离心500g 5分钟,弃上清,加入500LPBS上机或加入500 L 1% PFA固定后再上机 七、注意事项 1、标本处理 避免使用络合钙的抗凝剂,如ACD与EDTA,因为它们会限制依赖性激活过程,推荐用肝素钠。此外LPS,一种常见的生物试剂污染物,是强细胞激活

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