产品封面图

兔抗GAP-43多克隆抗体 多物种适用 gap43抗体

收藏
  • 询价
  • 2025年07月14日
  • ELISA;Immunoprecipitation
  • Rabbit
  • Mouse;Rat;Human;Bovine
avatar
默克生命科学
11 年金牌会员

  • 企业认证

    点击 QQ 联系

    • 相关产品推荐更多 >

    万千商家帮你免费找货

    0 人在求购买到急需产品
    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围：

      ELISA;Immunoprecipitation

    • 宿主：

      Rabbit

    • 适应物种：

      Mouse;Rat;Human;Bovine

    • 抗原来源：

      0

    • 库存：

      大量

    • 是否单克隆：

      2

    • 规格：

      100 μL

    Product Images

    Rabbit anti-GAP-43 (Catalog Number AB5220) staining of cultured neurons.

    Western Blot Analysis:
    Mouse brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Growth Associated Pr...
    Species Reactivity Key Applications Host Format Antibody Type
    H, M, R, Ch, B ELISA, IC, IH, IP, WB Rabbit Purified Polyclonal Antibody
    Description:
    Anti-Growth Associated Protein-43 (GAP-43)
    Background Information:
    GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.
    Alternate Names:
    Neuromodulin
    Specificity:
    Recognizes Growth Associated Protein-43 (GAP-43).
    Key Applications:
    • ELISA
    • Immunocytochemistry
    • Immunohistochemistry
    • Immunoprecipitation
    • Western Blotting
    Application Notes:
    Immunohistochemistry:
    1:1,000 dilution of a previous lot was used on 4% paraformaldehyde / PBS fixed or 3% Paraformaldehyde / 0.1% Gluteraldehyde / PBS fixed tissue sections.

    Immunocytochemistry:
    1:1,000 dilution of a previous lot was used on Hippocampal neurons in culture and PC12 cells stimulated with NGF.

    Immunoprecipitation:
    1:100 dilution of a previous lot was used.

    ELISA:
    1:2,000 dilution of a previous lot was used.
    Species Reactivity:
    • Human
    • Mouse
    • Rat
    • Chicken
    • Bovine
    Species Reactivity Note:
    Reacts to a high degree with mouse. Reacts to a lesser degree with rat, human, bovine and chicken. Other species have not been tested.
    Immunogen:
    Recombinant rat GAP-43 (complete sequence).
    Molecular Weight:
    23.6 kDa
    Control:
    Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation, cultured neurons.
    Presentation:
    Purified rabbit polyclonal in buffer containing PBS with 0.01% sodium azide as a preservative.
    Storage Conditions:
    Stable for up to 6 months at -20ºC in undiluted aliquots from date of receipt.
    Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
    Gene Symbol:
    • GAP43
    • PP46
    • B-50
    Quality Assurance:
    Routinely evaluated by Western Blot on Mouse brain lysates.

    Western Blot Analysis: 1:500 dilution of this lot detected Growth Associated Protein 43 on 10 μg of Mouse brain lysates.
    Antibody Category:
    Neuroscience
    Antibody Sub-Category:
    • Neuroregenerative Medicine
    • Signaling Neuroscience
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Format:
    Purified
    Host:
    Rabbit
    Concentration:
    Variable
    Antibody Type:
    Polyclonal Antibody

    更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com

    Recombinant rat GAP-43 (complete sequence). 详细描述见链接：http://www.millipore.com/catalogue/item/AB5220

    风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标文献和实验
    相关实验

    • 人脑神经相关生长蛋白-43(GAP-43)ELISA试剂盒 说明书

      人脑神经相关生长蛋白-43 ( GAP-43 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 GAP-43 单抗包被于酶标板上，标准品和样品中的 GAP-43与单抗结合，加入生物素化的抗人 GAP-43 ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸，在 450nm 处测 OD 值，GAP-43

    • 抗体指南：你知道做什么实验用什么抗体吗？

      用FACS 流式检测活细胞的表面蛋白，则需要选择含该表面蛋白的胞外域来免疫制备的抗体。 (2)样本的提取或处理过程：某些抗体要求样本经过某些特殊处理，例如：许多抗体只识别还原和变性的、表位已暴露不受二级四级结构阻碍的蛋白样本，另一方面，某些抗体仅识别天然折叠状态的蛋白。 当选择免疫组化的抗体时，应注意某些抗体只识别未固定的冷冻的组织，而另一些抗体则适用于无需抗原修复解交联步聚的甲醛固定石蜡包埋的组织，这些都会在抗体说明书上应用部分标示出来 3 样本的物种 应选择物种相同或有交叉反应的抗体，抗体

    • 抗体选择指南

      该表面蛋白的胞外域来免疫制备的抗体。 2. 样本的提取或处理过程：某些抗体要求样本经过某些特殊处理，例如：许多抗体只识别还原和变性的、表位已暴露不受二级四级结构阻碍的蛋白样本，另一方面，某些抗体仅识别天然折叠状态的蛋白。当选择免疫组化的抗体时，应注意某些抗体只识别未固定的冷冻的组织，而另一些抗体则适用于无需抗原修复解交联步聚的甲醛固定石蜡包埋的组织，这些都会在抗体说明书上应用部分标示出来。 三、样本的物种 应选择物种相同或有交叉反应的抗体，抗体可能与不同物种的同种靶蛋白有交叉反应，因其氨

    查看更多相关实验 >
    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    Anti-Growth Associated Protein 43; 100 μL
    询价
    默克生命科学
    2024年04月07日询价
    Growth Associated Protein 43 Antibody
    询价
    北京博蕾德生物科技有限公司
    2025年05月23日询价
    Polyclonal Antibody to Growth Associated Protein 43 (GAP43)
    询价
    安元生物科技（南京）有限公司
    2025年07月10日询价
    KD-Validated Anti-Growth associated protein 43 Rabbit Monoclonal Antibody #63163
    ￥650
    合肥善本生物科技有限公司
    2025年10月26日询价
    growth associated protein 43 Antibody 抗体，orb1742368，Biorbyt
    ￥7735
    武汉博欧特生物科技有限公司
    2025年07月07日询价
    兔抗GAP-43多克隆抗体 多物种适用 gap43抗体
    询价