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Anti-Sodium Channel, Voltage G

ated, Pan (SP19 Segment); 200 μL
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  • 询价
  • AB5210
  • 2026年01月01日
  • Western Blotting;Immunohistochemistry
  • Rabbit
  • Mouse;Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围：

      Western Blotting;Immunohistochemistry

    • 宿主：

      Rabbit

    • 抗原来源：

      0

    • 库存：

      大量

    • 适应物种：

      Mouse;Rat

    • 是否单克隆：

      2

    • 规格：

      200 μL

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    Western blotting of rat brain membranes:

    1. AB5210 (1:200)

    2. AB5210, preincubated with the control peptide antigen

    Rabbit anti-SP19 (Catalog Number AB5210) Staining in heart, atrial wall (inner side) of mouse. Photo courtesy of Dr. Shai Shoham, Herzog Hospital, Jerusalem.

    Rabbit anti-Pan Voltage Gated Sodium Channel, SP19 (Catalog Number AB5210). Staining of two myenteric ganglia with AB5210 (red) and an antibody to calbindin (green) which stained some of the neurons. Photo courtesy of Dr. W. Hartig, Leipzig University; Dr. M. Hanani, Hebrew University, Jerusalem.

    Species Reactivity Key Applications Host Format Antibody Type
    M, R IH, WB Rabbit Affinity Purified Polyclonal Antibody
    Description:
    Anti-Sodium Channel, Voltage Gated, Pan (SP19 Segment)
    Specificity:
    Recognizes alpha subunits of VGSC. Does not cross react with any other sodium channel antigens tested so far.
    Key Applications:
    • Immunohistochemistry
    • Western Blotting
    Application Notes:
    All procedures that are going to receive a full-length protein should be performed at 4°C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).

    Western blot: 1:200 using ECL on newborn rat brain membranes.

    Immunohistochemistry on mouse brain sections (frozen) and mouse spinal cord sections (paraffin).

    Dilutions should be made using a carrier protein such as BSA (1-3%)

    Optimal working dilutions must be determined by the end user.
    Species Reactivity:
    • Mouse
    • Rat
    Immunogen:
    Purified peptide corresponding to amino acids 1501-1518 in the alpha subunit (amino acids 1501-1518) of rat type I voltage-gated sodium channel (VGSC) (Accession P04774).
    Control:
    CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
    Presentation:
    Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
    Storage Conditions:
    Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
    Gene Symbol:
    • SCN1A
    • SCN1
    • HBSCI
    • Nav1.1
    • FEB3
    • NAC1
    • SMEI
    • GEFSP2
    Antibody Category:
    Neuroscience
    Antibody Sub-Category:
    • Ion Channels & Transporters
    • Neuronal & Glial Markers
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Therapeutic Areas:
    Inflammation
    Format:
    Affinity Purified
    Host:
    Rabbit
    Concentration:
    1.0 mg/mL (after reconstitution)
    Antibody Type:
    Polyclonal Antibody

    更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com

    Purified peptide corresponding to amino acids 1501-1518 in the alpha subunit (amino acids 1501-1518) of rat type I voltage-gated sodium channel (VGSC) (Accession P04774). 详细描述见链接：http://www.millipore.com/catalogue/item/AB5210

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