- 询价
- AB5154-200UL
- 2025年12月31日
- Immunoprecipitation;Western Blotting
- Rabbit
- Rat
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- 文献和实验
- 技术资料
- 应用范围：
Immunoprecipitation;Western Blotting
- 宿主：
Rabbit
- 适应物种：
Rat
- 抗原来源：
0
- 库存：
大量
- 是否单克隆：
2
- 规格：
200 μL
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | IC, IP, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Cav2.2
Alternate Names:
Voltage Gated Calcium Channel (N-type) alpha 1B Subunit; Cacna1b
Specificity:
Recognizes a 1B subunit (both LMW and HMW forms) of voltage-gated calcium channel. Does not cross react with any other calcium channel antigens tested so far.
SPECIES REACTIVITIES: It is expected that the antibody will also work on mouse due to sequence homology (15/17). Other species have not been tested.
SPECIES REACTIVITIES: It is expected that the antibody will also work on mouse due to sequence homology (15/17). Other species have not been tested.
Key Applications:
- Immunocytochemistry
- Immunoprecipitation
- Western Blotting
Application Notes:
In preparing full-length protein samples for Western blot or immunoprecipitation, the procedures should be performed at 4°C, with the following protease inhibitor mixture: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).
Immunoprecipitation
Immunocytochemistry. Recommend subsequent staining with a labeled second antibody.
Western blot: 1.5 to 3.0 μg/mL (1:100-1:200) using ECL
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Immunoprecipitation
Immunocytochemistry. Recommend subsequent staining with a labeled second antibody.
Western blot: 1.5 to 3.0 μg/mL (1:100-1:200) using ECL
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Species Reactivity:
Rat
Immunogen:
Purified peptide CNB1 from 851-867 of alpha 1B subunit of rat brain voltage-gated calcium channel (Accession Q02294; Westenbroek et al. 1992) containing N-terminal lysine and tyrosine, conjugated to KLH with gluteraldehyde (Harlow & Lane 1988).
Control:
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 10 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation:
Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Storage Conditions:
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at 2-8°C in undiluted aliquots for up to 2 weeks. For longer periods, it is recommended to add glycerol (ACS grade or better) at a ratio of 1:1 and store the antibody at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Gene Symbol:
- CACNA1B
- BIII
- CACH5
- Cav2.2
- CACNN
- CACNL1A5
Antibody Category:
Neuroscience
Antibody Sub-Category:
- Ion Channels & Transporters
- Neuroinflammation & Pain
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Therapeutic Areas:
Oncology
Format:
Affinity Purified
Host:
Rabbit
Concentration:
0.3 mg/mL (after reconstitution)
Antibody Type:
Polyclonal Antibody
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Purified peptide CNB1 from 851-867 of alpha 1B subunit of rat brain voltage-gated calcium channel (Accession Q02294; Westenbroek et al. 1992) containing N-terminal lysine and tyrosine, conjugated to KLH with gluteraldehyde (Harlow & Lane 1988). 详细描述见链接：http://www.millipore.com/catalogue/item/AB5154-200UL
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文献和实验相关实验
在 WB 和 IP 实验中，破碎细胞或组织与选择和配制裂解液一样重要。比起一些更软的组织（如脑组织），在准备 WB 或 IP 的样品时，紧密的纤维组织（如肌肉组织）需要更剧烈的方法；对于培养的原代细胞或细胞系，在准备 WB 和 IP 的样品时需要一定的机械搅拌去提取蛋白。机械破碎样品常规的方法和设备如下：组织高速匀浆仪通常破碎体积大的植物或者动物组织需要用可旋转、带有刀片的匀浆仪或者搅拌器 .这些仪器通常通过一个电动的马达带动不锈钢可旋转切割刀片，可以从顶部或者底部启动，这个过程中产生很少热量
原理、优缺点、适用场景以及实操避坑要点。 一、免疫共沉淀Co-IP（细胞内复合物验证） 核心原理 在非变性条件下裂解细胞，尽量保留原有蛋白复合物。利用靶蛋白特异性抗体，将靶蛋白及在当前裂解条件下仍与其形成复合物的蛋白共同富集。洗脱后既可以用Western blot验证已知候选蛋白，也可以联合质谱（Co-IP-MS）筛选潜在互作分子。 关键点： Co-IP可以证明两个蛋白在细胞内存在于同一复合物中，但无法区分是直接物理结合，还是由第三个蛋白或其他分子介导的间接关联。 适用
。 实验类型：大多数只标明用于WB、IP、IHC、IF、ChIP的抗体都不适用于流式实验(FC,flow cytometry)。选择标明用于FC实验的抗体，并有引用文献和实验数据图。 克隆号：有一些标记物有多个克隆号，选择文献报道中使用多，荧光标记种类多的克隆号。 厂家和批号：不同厂家或同一厂家生产的不同批号的相同荧光的相同标记物的抗体，荧光染料的强度可能有少许差别，不建议一次实验混合使用。 2、流式抗体荧光标记的方式包括直接标记和间接标记两种。 在流式实验过程中，直标抗体一抗上连接有荧光染料，间标
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Anti-Cav2.2 兔多克隆亲和纯化抗体 大鼠适用 IP/WB
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