产品封面图

Chemicon Anti-D1A受体(C端胞质) 兔多克隆

亲和纯化 ELISA/WB/IH(猪/大鼠)
收藏
  • 询价
  • AB1765P
  • 2025年12月27日
  • ELISA;Western Blotting
  • Rabbit
  • Pig;Rat
avatar
11金牌会员
  • 企业认证

    点击 QQ 联系

    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围

      ELISA;Western Blotting

    • 宿主

      Rabbit

    • 适应物种

      Pig;Rat

    • 库存

      大量

    • 抗原来源

      0

    • 是否单克隆

      2

    • 规格

      50 μg

    Species Reactivity Key Applications Host Format Antibody Type
    Po, R ELISA, IH, WB Rabbit Affinity Purified Polyclonal Antibody
    Description:
    Anti-Dopamine D1A Receptor, C-terminus, cytoplasmic
    Specificity:
    Dopamine Receptor D1A. An antibody made to the D1R peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes by Western blot. The 75 kDa band was not visible in striatum (Huang et al. 1992). Approximately 100-400 μg of protein/lane should be loaded to visualize the 75 kDa band. By immunohistochemistry D1R has been localized in various regions of the rat brain (Huang et al. 1992).

    May recognize porcine or bovine (100 % similarity) or human (78% similarity).
    Key Applications:
    • ELISA
    • Immunohistochemistry
    • Western Blotting
    Application Notes:
    Western blot: 1-10 μg/mL (Chemiluminescence technique). An antibody made to this peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes. The 75 kDa band was not visible in striatum (Huang et al. 1992; O'Connel et al. 1995). Approximately 100-400 μg of protein/lane should be loaded to visualize the 75 kDa band (Huang et al. 1992; O'Connel et al. 1995) that is present in relatively small amounts.

    Immunohistochemistry: 2-10 μg/mL on paraformaldehyde fixed sections.

    ELISA: 0.5-1.0 μg/mL (1 μg/mL D1A peptide (Cat. Number AG259)/well)

    Optimal working dilutions must be determined by end user.
    Species Reactivity:
    • Pig
    • Rat
    Immunogen:
    A 13 amino acid peptide sequence (amino acids 403-415) from rat D1A (Monsma et al. 1990; Zhou et al. 1990; Zhou et al. 1992; O'Dowd et al. 1990). This peptide is predicted to be cytoplasmic.
    Epitope:
    C-terminus, cytoplasmic
    Presentation:
    Affinity purified immunoglobulin. Liquid in 100mM Tris, pH 7.5 with 0.2% BSA and 0.05% sodium azide.
    Storage Conditions:
    Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
    Gene Symbol:
    • DRD1
    • DRD1A
    • DADR
    • D
    Antibody Category:
    Neuroscience
    Antibody Sub-Category:
    Neurotransmitters & Receptors
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Format:
    Affinity Purified
    Host:
    Rabbit
    Concentration:
    0.5 mg/mL
    Antibody Type:
    Polyclonal Antibody

    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    A 13 amino acid peptide sequence (amino acids 403-415) from rat D1A (Monsma et al. 1990; Zhou et al. 1990; Zhou et al. 1992; O'Dowd et al. 1990). This peptide is predicted to be cytoplasmic. 详细描述见链接:http://www.millipore.com/catalogue/item/AB1765P

    风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标文献和实验
    相关实验
    • 如何用外泌体「套路」申请关于国自然?

      Tracking Analysis),该技术已被外泌体研究领域认可为外泌体表征手段之一。NTA 技术的样本处理简单、能保证外泌体原始状态、检测速度快,检测后能提供外泌体粒径和浓度信息。 图五 外泌体 NTA 图 Western Blot 分子标志物检测: WB 可以用来鉴定外泌体的标志蛋白是否存在于样本中。外泌体标志蛋白包括四跨膜蛋白家族,如 CD9、CD63 和 CD81;细胞质蛋白,如肌动蛋白(Actin)和钙磷脂结合蛋白(Annexins);参与生物功能的分子,如凋亡转接基因 2 互作蛋白 X

    • 蛋白印迹(Western Blot)常用试剂

        WB010 蛋白酶+磷酸酶抑制剂混合物(20X) 1ml 380   WB011 流式用破膜剂 50T 1300 Invitrogen原装 WB012 淋巴细胞分离液(小鼠、大鼠等) 250ml

    • 【资源】Western Blot 简略而细致

      )、ELISA亲和纯化、免疫荧光及免疫组化。注:因为GAPDH 作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的。在实验中,可能存在总蛋白浓度测定不准确;或者蛋白质样品在电泳前上样时产生的样品间的操作误差;这些误差需要通过测定每个样品中实际转到膜上的GAPDH的含量来进行校正,所以一般的western实验都需要进行内参设置。具体校正的方法就是将每个样品测得的目的蛋白含量与本样品的GAPDH含量相除,得到每个样品目的蛋白的相对含量。然后才进行样品与样品之间的比较。反应:抗GAPDH单抗(clone

    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    询价
    默克生命科学
    2025年07月15日询价
    ¥4729
    苏州瑞诺德生物科技有限公司
    2025年05月18日询价
    ¥4275
    北京智杰方远科技有限公司
    2025年05月21日询价
    ¥5002.50
    瑞博奥(广州)生物科技股份有限公司
    2025年07月11日询价
    ¥3057
    西宝生物科技(上海)股份有限公司
    2025年06月04日询价
    Chemicon Anti-D1A受体(C端胞质) 兔多克隆 亲和纯化 ELISA/WB/IH(猪/大鼠)
    询价