Anti-Iron regulatory protein 1

Anti-Iron Pegulatory Protein 1

Iron regulatory proteins (IRP-1 and IRP-2) are cytoplasmic mRNA-binding proteins that control intracellular iron levels by regulating the translation of ferritin, Tfrs, and other proteins congaing iron-responsive element (IRE) located at the 5'-UTR. IRP binds to IREs with high affinity in situation of iron starvation. Binding of IRP to IRE of ferritin represses its transcription. However, when cells are iron replete, IRPs lose their capacity to bind IREs, allowing efficient translation of ferritin and reducing Tfr mRNA half-life. Although IRP1 and IRP-2 share significant protein sequence homology, they differ in tissue distribution and mode of regulation. IRP-2/IRE-BP2 (rat/human 963 aa) is relatively less abundant than IRP-1 and lacks aconitase activity. It has a unique 73-aa insertion in the N-terminus. In the presence of high iron levels, IRP-2 is rapidly targeted to proteasome-mediated degradation. Although, both IRPs bind the consensus IREs, it is also shown that IRP-2 can recognize an exclusive IRE subset.

IRP-1

Iron regulatory protein 1 (IRP-1). The immunogen sequence has no significant homology with IRP-2.

• ELISA
• Western Blotting

Western blot: 1-10 µg/mL using ECL.
Immunohistochemistry: Not tested. Recommended testing antibody dilution:2-20 µg/mL
ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide/well.
Optimal working dilutions must be determined by end user.

Rat

Other species have not yet been tested. The immunogen sequence is 94% conserved in mouse, and 89% in human and rabbit IRP-1.

Synthetic peptide near the N-terminus of rat IRP-1.

Liquid in PBS with 0.1% BSA.

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

• ACO1
• Aconitase
• IREBP
• IRP1
• OTTHUMP00000045233
• IREB1
• EC 4.2.1.3

Neuroscience

• Neurodegenerative Diseases
• Neurofilament & Neuron Metabolism
• RNA Binding Protein (RBP)

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Chemicon (Millipore)

Rabbit

Polyclonal Antibody