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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
Cell Culture
- 宿主:
0
- 适应物种:
All
- 抗原来源:
0
- 库存:
大量
- 是否单克隆:
0
- 规格:
20 mL
Surface area of plates: (pi)(Radius squared) in cm(2)
In partical terms working dilutions of 50μg/mL to 100μg/mL are usually adequate.
Dilute poly-D-lysine in PBS, water, or 0.1M borate buffer {prepared by adding 1.24 g boric acid and 1.9 g sodium tetraborate in 400 ml water, pH 8.5} to final working concentration.
Plates or flasks are coated for 3 hours to overnight, and up to 3 days if stored at 4C. Best results are usually with just over night coatings.
Remove poly-d-lysine solution and rinse with sterile PBS or water 1-2X. Do not scrape the bottom of the coated plates or flasks.
Use plates immediately or store with PBS for up to 5 days.
Poly-D-lysine coating methods will vary slightly from laboratory to laboratory, please use the method as a general guide.
- Human Embryonic Stem Cells
- Mesenchymal Stem Cells
- Neural Stem Cells
- Hematopoietic Stem Cells
- Epithelial Cells
- Pancreatic Stem Cells
- Induced Pluripotent Stem Cells
- Stem Cell Reagents
- Attachment Factors
Product Family Information
ECM Proteins and coated platesMillipore offers a wide variety of ECM proteins and coated cultureware to meet the individual needs of your cell line. Our ECM Cell Culture Optimization Array can help you determine the best coating and concentration for your particular cell type. |
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
详细描述见链接:http://www.millipore.com/catalogue/item/A-003-E
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Oxalyticase Dissolve in 0.1 M NaCl, 0.02% Na azide, 50% glcyerole Store aliquots (1 ml) at 20o C in common freezer for stock solutions 上一篇:ALPHA FACTOR ARREST AND RELEASE 下一篇:GLASS BEADS
Ampicillin (50mg/ml) Ampicillin Dissolve Ampicillin (Na salt from Sigma, A-9518) in H2 0. Store 1 ml aliquots at 20o C in common freezer for stock solutions 上一篇:PREPARATION
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