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Poly-D-Lysine solution, 1.0 mg

/mL; 20 mL
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  • 询价
  • A-003-E
  • 2025年12月24日
  • Cell Culture
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围

      Cell Culture

    • 宿主

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    • 适应物种

      All

    • 抗原来源

      0

    • 库存

      大量

    • 是否单克隆

      0

    • 规格

      20 mL

    Description:
    Poly-D-Lysine solution, 1.0 mg/mL
    Product Overview:
    Poly-D-Lysine promotes the adhesion of tissues/sections to the culture vessel. The polycationic property of the molecule allows interaction with anionic sites on the tissue/cells promoting effective attachment to the growing surface. The molecular weight of Poly-D-Lysine is >300,000 Da.
    Key Applications:
    Cell Culture
    Application Notes:
    The level of coating will vary for different species and types of cells. In general final coating concentrations from 3-10μg/cm(2) of surface area will typically supply sufficent coatings for most cell types.



    Surface area of plates: (pi)(Radius squared) in cm(2)



    In partical terms working dilutions of 50μg/mL to 100μg/mL are usually adequate.



    Dilute poly-D-lysine in PBS, water, or 0.1M borate buffer {prepared by adding 1.24 g boric acid and 1.9 g sodium tetraborate in 400 ml water, pH 8.5} to final working concentration.

    Plates or flasks are coated for 3 hours to overnight, and up to 3 days if stored at 4C. Best results are usually with just over night coatings.

    Remove poly-d-lysine solution and rinse with sterile PBS or water 1-2X. Do not scrape the bottom of the coated plates or flasks.

    Use plates immediately or store with PBS for up to 5 days.



    Poly-D-lysine coating methods will vary slightly from laboratory to laboratory, please use the method as a general guide.
    Species Reactivity:
    All
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Cell Culture Media or Reagent Type:
    Attachment Factors
    Stem Cell Type:
    • Human Embryonic Stem Cells
    • Mesenchymal Stem Cells
    • Neural Stem Cells
    • Hematopoietic Stem Cells
    • Epithelial Cells
    • Pancreatic Stem Cells
    • Induced Pluripotent Stem Cells
    Stem Cell Workflow Stage:
    Maintenance & Expansion
    Presentation:
    Liquid. 20ml in sterile water.
    Storage Conditions:
    Store at -20C for upto 18 months.
    Cell Culture Reagent Type:
    • Stem Cell Reagents
    • Attachment Factors
    Alternate Names:
    poly lysine

    Product Family Information

    ECM Proteins and coated plates

    Millipore offers a wide variety of ECM proteins and coated cultureware to meet the individual needs of your cell line. Our ECM Cell Culture Optimization Array can help you determine the best coating and concentration for your particular cell type.

    » More…


    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    详细描述见链接:http://www.millipore.com/catalogue/item/A-003-E

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