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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Prokaryotic recombinant protein corresponding to a portion of the C-terminus of the mature human nitric oxide synthase-1 protein.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX01921
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
IHC-P
- 浓度:
>27 μg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
nNOS
- 抗体英文名:
nNOS antibody [NOS-125]
- 抗体名:
nNOS 抗体 [NOS-125]
- 规格:
500 μl
IHC-P analysis of human small intestine using GTX01921 nNOS antibody [NOS-125]. Note cytoplasmic staining of enteric ganglia.
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文献和实验Cloning and Expression of Human eNOS and nNOS Using the Baculovirus-Insect Cell System
To date, the cDNAs and genes encoding three distinct forms of the enzyme nitric oxide synthase (NOS) have been described (reviewed in ref. 1 ). The family of NOS enzymes generate nitric oxide (NO) from arginine, producing citrulline
Detection of NOS Isoforms by Western-Blot Analysis
The amount of nitric oxide synthase (NOS) in tissues and cells can readily be determined by Western blotting. Antibodies are now commercially available for the detection of the inducible (i), neuronal (n), and endothelial (e) NOS
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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