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文献和实验agarose as possible, and place in a preweighed and labeled eppendorf tube. 9. Weigh the agarose gel slice, and add 3 volumes of buffer QG (from the Qiagen Gel Extraction Kit). Incubate at 42 ℃ for 10-15 min until the gel slice is completely dissolved
Fastfilter Plasmid Maxi Kit Spin Protocol
conditions of bacteria is vital of obtaining maximal plasmid DNA yields. The best conditions are achieved by picking a single isolated colony from a freshly transformed or freshly plate to inoculate a 2-5ml starter culture containing the appropriate
Fastfilter Plasmid Midi Kit Spin Protocol
growth conditions of bacteria is vital of obtaining maximal plasmid DNA yields. The best conditions are achieved by picking a single isolated colony from a freshly transformed or freshly plate to inoculate a 2-5ml starter culture containing
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