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- 2025年12月20日
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文献和实验, as determined in the previous procedure. Wash the plate four times in PBS/Tween (0.05%). Add streptavidin-peroxidase conjugate (diluted 1 in 5000 in PBS/BSA (1%)) to all of the wells. Incubate
-PAGE, transferred to nitrocellulose, and detected by the addition of streptavidin-enzyme conjugate followed by substrate. ECM Protocols Western Blot (LTI) A mixture of protein is separated electrophoretically by SDS-PAGE, and the individual protein
monoclonal anti-DIG (1/400 dilution of a 1 mg/ml stock solution in PBTB; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA; Catalog No. 200-062-156) and Streptavidin-HRP conjugate (1/100 dilution of a 1 m g/mL stock solution in PBTB; Molecular
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