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- 2026年01月02日
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文献和实验Large Scale Plasmid Preps: PEG method
and inspect on agarose gel. 14. Store glycerol stocks of the bacteria and make plasmid maps if they are not already done. SOLUTIONS: Solution II: 0.2M NaOH, 1% SDS. (Make fresh. For 20 mL add 0.4 mL 5M NaOH, plus 2 mL 10%SDS)
Large Scale Plasmid Preps: Qiagen/Cesium Method
most of it will pellet along with the bacterial cell wall. However the relative purity of the plasmid DNA will drop if the plasmid is large or at a relatively low copy number in the prep. The combination of a kit prep with cesium banding eliminates this problem
Pick colonies into 0.5ml of SD-Leu (or other appropriate SD medium) Vortex for 1min Leave to grow O/N for 18-24h at 30°C, 230-250rpm (best in 5ml bijou) Spin yeast culture at 13,000rpm for 5 min (microfuge) Pour
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