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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 英文名:
Mesenchymal Stem Cell Adipogenic Differentiation Medium
- 供应商:
上海觅拓生物
- 规格:
500ml
间充质干细胞-脂肪细胞分化培养基描述:
Our adipogenesis differentiation medium has been specifically developed and optimized for in vitro mesenchymal stem cell adipogenesis study. Generally, with this medium, we obtain >35% mature adipocytes from human bone marrow, >50% from rat bone marrow, and >60% from human mesenchymal stem cell isolated from adipose tissue. Efficiency of adipogenic differentiation depends on the quanlity of the mesenchymal stem cells. Mesenchymal stem cell Adipogenic Differentiation Medium (MADM) is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. Components MADM consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of mesenchymal stem cell adipogenic differentiation supplement (MADS, Cat. No. 7542-5), 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503). Note: MADS may be slightly hazy; Haziness does not affect cell culture and differentiation efficiency.
MADM (500ml) consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of mesenchymal stem cell osteogenic differentiation supplement (MODS, Cat. No. 7542-5), 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).
Note: MADS may be slightly hazy; Haziness does not affect cell culture and differentiation efficiency。
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文献和实验Adipogenic Differentiation of Human Mesenchymal Stem Cells
Mesenchymal stem cells have the capability to differentiate into a number of cell types including adipocytes. The adipocytic phenotype is characterized by intracellular accumulation of lipid droplets as well as transcription of adipocyte
Adipogenic Differentiation of Adipose-Derived Stem Cells
to undergo adipogenic differentiation in vitro by exposure to a cocktail of chemical agents or inductive growth factors. The current chapter describes methods to induce adipogenesis and to quantify this differentiation process in vitro.
Adipogenic Differentiation of Human Induced Pluripotent Stem Cells
Human induced pluripotent stem (iPS) cells are promising sources of disease modeling and regenerative medicine. However, differentiation properties of human iPS cells to specific lineages still remain to be fully clarified
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