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- 上海
- FSM0010-100ML
- 2025年07月16日
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- 库存：
20
- 供应商：
上海觅拓生物
- 规格：
100ml
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文献和实验Making Boiled Donkey (or whatever) Serum (BDS) for Blocking
anything your antibodies will bind in serum will bind before you put on the secondary. 1) Boil normal donkey serum in the portion of ddH2O required to make a 5% serum solution in PHEM (which you'll add from your 2x stock after boiling) for 10 minutes. Note: use caution
IF protocol for perfusion fixed tissue
Wash free floating brains sections 4 times in 0.01M phosphate buffer saline (PBS). (The longer the sections are washed the better) To quench non-specific binding, incubate sections in 10 v/v normal donkey serum (in PBS-triton 0.3 v/v (PBST
Wholemount staining of embryos
5'' washes with TBS. Use haematology mixer (these are really good mixers Fisher cat#14-060-1) Block protein binding sites by incubating embryos in TBS plus 2 mg/ml BSA plus o.1 (v/v) triton-X-100 plus 20 heat inactivated normal serum (donkey or goat
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