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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
NORMAL DONKEY SERUM, STERILE
- 供应商：
北京智杰方远科技
- CAS号：
******
- 保存条件：
详见说明
- 规格：
****
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文献和实验Making Boiled Donkey (or whatever) Serum (BDS) for Blocking
anything your antibodies will bind in serum will bind before you put on the secondary. 1) Boil normal donkey serum in the portion of ddH2O required to make a 5% serum solution in PHEM (which you'll add from your 2x stock after boiling) for 10 minutes. Note: use caution
The outer layer of human skin is the epidermis. More than 90% of the epidermal cells are keratinocytes, which originate from stem cells, located in the basal cell layer (Stratum basale ). Keratinocytes in the basal layer divide, differentiate
inactivate by bringing to 56°C for 30 min. 8. Serum can be used immediately or stored at -20°C (serum is not sterile at this point!). Do not store at 4°C unless filter sterilzed.
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