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上海觅拓生物
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文献和实验low frequency table Codon usage for C. elegans lowly biased (lowly expressed, by inference) genes. (Stenico, M., Lloyd, A. T., and Sharp, P. M. (1994). Nucleic Acids Research 22: 2437-2246 AmAcid
3/4 to 1 hr at 37ºC. I usually plate the whole transformation after spinning down the cells in a low speed centrifuge at 2000 rpm for 5 minutes. If I ligate 100 ng of blunted dephosphorylated vector to 50 ng of a blunt 500 bp PCR in a 30 µl reaction, I'll
Preparation of low density, collagenase-digested splenocytes Protocol
Preparation of low density, collagenase-digested splenocytes Protocol 1. Dilute 2 ml of 4000 U/ml Collagenase D as follows: 1 ml into 9 ml HBSS/Ca++/Mg++ (=400U/ml) and 1 ml into 39 ml HBSS/Ca++/Mg++ (=100U/ml). Put on ice
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