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- 库存:
20
- 供应商:
上海觅拓生物
- 规格:
10ml
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文献和实验引物:5’-TGCACCATCTGTCACTCTGTTAACCTC-3’ 2) PCR扩增 (1) 在冰浴中对各样品制备PCR重要混合物,对每个样品均加入: 10X PCR缓冲液 5.0ml 25mmol/L dNTPs 混合物 0.4ul 40umol/L 16sr DNA引物 每种引物1.0ul 40umol/L pBR322 DNA 引物 每种 2.0ul pBR322 DNA 1pg/ml 2.0ul DNA聚合酶(5U/ml) 0.2ul 三蒸水 35.4ul 总量 45ul (2) 用无菌去离子水稀释样品为1:10
and mycobacteria, leaving tissue culture cells free of their awful influence. Two hundred-milligram Cipro is diluted from 10 mg/mL stock to 10 uL/mL (i.e. 1:1000) in complete F-12 medium (including both FBS and the usual pen/strep/amp mixture). Before introduction
, leaving tissue culture cells free of their awful influence. Two hundred-milligram Cipro is diluted from 10 mg/mL stock to 10 uL/mL (i.e. 1:1000) in complete F-12 medium (including both FBS and the usual pen/strep/amp mixture). Before introduction of drug
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