密理博抗体 生产蛋白质抗体

BrdU IHC Kit

Chemicon (Millipore)

Test Principle

CHEMICON®'s BrdU Immunohistochemistry Kit is a non-isotopic immuno-histochemistry staining kit for the detection and localization of BrdU incorporated into newly synthesized DNA of proliferating cells. Visual staining of the cells is achieved by using a biotinylated monoclonal antibody to BrdU followed by streptavidin-HRP conjugate and DAB substrate.

Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has been a traditional method for detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting of autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment.

A well established alternative to [3H] thymidine uptake has been demonstrated by numerous researchers. In these methods bromodeoxyuridine (BrdU), a thymidine analog, replaces [3H] thymidine. BrdU is incorporated into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells which are synthesizing DNA.

Immunohistochemistry (Paraffin)

The CHEMICON® Kit is designed for in vitro detection of BrdU in proliferating cells. The material in this kit is sufficient to run 50 slides. The average test area is defined as a circle around the tissue with an approximate diameter of 2 centimeters.

For Research Use Only. Not for use in diagnostic procedures.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

• IHC Detection Kits
• Cell Proliferation Assays
• Immunohistochemistry Assays

• 4X Trypsin Enzyme Concentrate*: (Part No. 2760a) One 3 mL bottle.
  
  *Tryspin is only required if you are using formalin fixed tissues. If the tissues are fixed in alcohol, trypsin digestion is not necessary.
• Trypsin Dilution Solution*: (Part No. 2760b) One 12 mL bottle.
• Denaturing Solution: (Part No. 2760c) One 6 mL bottle.
• Blocking Buffer: (Part No. 2760d) One 6 mL bottle.
• Detector Antibody (Biotinylated and ready-to-use): (Part No. 2760e) One 6 mL bottle.
• Streptavidin-HRP Conjugate (ready-to-use): (Part No. 2760f) One 6 mL bottle.
• Substrate Reaction Buffer: (Part No. 2760g) One 6 mL bottle.
• DAB Concentrate: (Part No. 2760h) One 0.3 mL.
• Hematoxylin Counterstain: (Part No. 2760i) One 6 mL bottle.
• Mounting Media: (Part No. 2760j) One 6 mL bottle.
• 5 Control Slides: Intestinal tissue from mouse injected with BrdU. 1 Positively stained (Part No. 2760k) and 4 unstained (Part No. 2760l).

Maintain the unopened kit at -20°C for up to 6 months after date of receipt. After opening the kit it may be stored at 2° to 8°C for 4-5 days. For long-term storage it is recommended that you aliquot each component and freeze at -20°C for up to 3 months after opening. Is it recommended that you wear latex or rubber gloves when running this kit, especially avoid contact with the DAB reagent.

1. Quenching Solution: Hydrogen peroxide (30%) solution for quenching endogenous peroxidase activity.

2. Phosphate buffered saline (PBS) solution.

3. Distilled water.

4. Ethyl alcohol

5. Xylene

6. Coverslips

7. Bromodeoxyuridine (BrdU)

8. Methanol