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IHC Select® Proteinase K; 15 m

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  • 询价
  • 21627
  • 2025年12月17日
  • Immunohistochemistry (Paraffin)
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 应用范围

      Immunohistochemistry (Paraffin)

    • 宿主

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    • 库存

      大量

    • 抗原来源

      0

    • 是否单克隆

      0

    • 规格

      15 mL

    Description:
    IHC Select® Proteinase K
    Trade Name:
    • IHC Select
    • Chemicon (Millipore)
    Key Applications:
    Immunohistochemistry (Paraffin)
    Application Notes:
    For use as a Pretreatment Reagent in Immunohistochemical staining procedures. Use Proteinase K to retrieve Epitopes in formalin-fixed, paraffin-embedded tissue sections mounted on glass microscope slides. This procedure is performed after deparaffinization and prior to IHC.



    Warm to room temperature before use. Digestion time and dilution should be determined by user as concentration is dependent on the type of antibody, antigen, and fixation method used. Recommended starting dilution range: Neat to 1:30.



    For best results use this reagent in conjunction with other IHC Select® detection reagents. A complete list of reagents is available at www.chemicon.com.
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Kit or Assay Type:
    Immunohistochemistry Assays
    Presentation:
    Liquid Proteinase K containing 15mM sodium azide as a preservative. Discard if it becomes cloudy or contaminated.
    Storage Conditions:
    When stored at 2-8°C, the Proteinase K is stable up to the expiration date printed on the label. Do not freeze or expose to elevated temperatures.
    Packaging:
    200 μg/mL
    Reagent Type:
    • Protein Reagents
    • Immunohistochemistry Reagents
    Alternate Names:
    Antigen/ Epitope Retrieval Reagent

    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    详细描述见链接:http://www.millipore.com/catalogue/item/21627

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    • 蛋白酶K(Proteinase K)使用小贴士

      分子量: 28.9 kDa 单位定义: 在250 μl反应体系, 37℃条件下1分钟内能分解释放相当于1 μmol酪氨酸的folin-阳性氨基酸和肽所需要的蛋白酶K的量定义为一个单位(1) 贮存条件: 20 mM Tris-HCl (pH 7.4), 1 mM CaCl2和50%甘油。贮存于-20℃   Proteinase K,中文名为蛋白酶K。不含DNase,不含

    • Mag-Bind Blood DNA Maximum Yield Protocol (2ml-6ml)

      实验步骤   1. Place a empty, uncapped 50 ml conical tube into a 50ml tube holder. 2. Add 1-6 ml blood sample to 50ml tube. 3. Add indicated volume of PBS, MSL, Proteinase K and RnaseA showed in the table to the sample. Mix

    • Mag-Bind Blood DNA Maximum Yield Protocol (7ml-10ml)

      . 3. Add indicated volume of PBS, MSL, Proteinase K and RnaseA showed in the table to the sample. Mix throughly by vortexing. 4. Incubate sample at 65°C for 20 min. Briefly vortex the tube few times during incubation. Cool the sample to room

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