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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
Chromatin Immunoprecipitation
- 宿主:
0
- 库存:
大量
- 抗原来源:
0
- 是否单克隆:
0
- 规格:
10 mL
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
详细描述见链接:http://www.millipore.com/catalogue/item/20-163
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文献和实验ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
to get through these as quickly as possible. 1. Spin down beads @10K, 1min, RT. 2. Aspirate supernatant. 3. Wash with 1ml of the following buffers and mix by inverting 3 or 4 times. Only aspirate supernatant to 0.1ml marking so no beads are lost: o FA lysis buffer
10X TBE BUFFER 3 Liters 324 g Tris base 165g Boric acid 120mls 0.5M EDTA (pH 8.0) autoclave for 20 min 2 Liters 216g Tris base 110g Boric acid 80mls 0.5M EDTA (pH 8.0) autoclave
Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer
L PMSF solution, 10 μL sodium orthovanadate solution and 10 μL protease inhibitor cocktail solution to 1ml of 1X RIPA buffer to prepare complete RIPA Lysis buffer Pour off media from tissue culture dish into waste container Wash
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