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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 应用范围:
Immunoprecipitation;Western Blotting
- 宿主:
0
- 抗原来源:
0
- 库存:
大量
- 是否单克隆:
0
- 规格:
100 mL
- Immunoprecipitation
- Western Blotting
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
详细描述见链接:http://www.millipore.com/catalogue/item/20-188
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- 作者
- 内容
- 询问日期
文献和实验Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer
L PMSF solution, 10 μL sodium orthovanadate solution and 10 μL protease inhibitor cocktail solution to 1ml of 1X RIPA buffer to prepare complete RIPA Lysis buffer Pour off media from tissue culture dish into waste container Wash
10X TBE BUFFER 3 Liters 324 g Tris base 165g Boric acid 120mls 0.5M EDTA (pH 8.0) autoclave for 20 min 2 Liters 216g Tris base 110g Boric acid 80mls 0.5M EDTA (pH 8.0) autoclave
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer Day -2: o Start a 5ml overnight culture from a single colony. Day -1: o Start a larger overnight culture using part of the 5ml culture (subculture). Day
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