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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
Chromatin Immunoprecipitation
- 宿主:
0
- 库存:
大量
- 抗原来源:
0
- 是否单克隆:
0
- 规格:
24 mL
详细描述见链接:http://www.millipore.com/catalogue/item/20-156
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文献和实验Immunoprecipitation and Immune Complex kinase assay
Immunoprecipitation and Immune Complex kinase assay--according to Tamara Hurley 1) In the cold room, wash cells with cold "Tris" (the same stuff used for TC). Spin down the cells if you are working with suspension cells in a low speed
Immunoprecipitation and Immune Complex kinase assay--according to Tamara Hurley
. Wash on plate if adherent cells by aspirating medium,adding 2 to 10 ml Tris,and aspirating again. 2)Lyse cells at 107 cells/ml or (minimally)500 microliters/10 cm dish for adherent cells in lysis buffer (e.g.NP-40,RIPA). Gently resuspend
Immunoprecipitation and Immune Complex kinase assay--according to Tamara Hurley
Immunoprecipitation and Immune Complex kinase assay--according to Tamara Hurley 1) Wash cells with cold "Tris" (from Margie's shelf). Spin down the cells if you are working with suspension cells in a low speed swinging
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