TeSR™-E8™3D is an animal component-free culture medium developed for the expansion and scale-up of undifferentiated human embryonic stem (ES) and induced pluripotent stem (iPS) cells as aggregates in 3D suspension culture.
Based on TeSR™-E8™, TeSR™-E8™3D is a low-protein medium with an optimized protocol for scaling up human ES and iPS cell production in suspension culture. The novel fed-batch workflow saves time and media; daily feeds replenish nutrients and eliminate the need for medium exchanges on non-passaging days.
TeSR™-E8™3D is made with the highest level of quality and care. It contains only the essential components required for scale-up of ES and iPS cells. The TeSR™-E8™3D system is compatible with cells previously cultured in mTeSR™1 or TeSR™-E8™ on Corning® Matrigel® hESC-Qualified Matrix (Corning Catalog #354277) or Vitronectin XF™ (Catalog #07180, a matrix developed and manufactured by Nucleus Biologics), and is compatible with a range of suspension culture vessels.
Advantages: • OPTIMIZED: Part of the TeSR™ family, mTeSR™3D and TeSR™-E8™3D are optimized for hPSC suspension culture
• STREAMLINED WORKFLOW: Fed-batch strategy provides a simplified culture system
• SIMPLIFIED CULTURE SYSTEM: Serum-free culture system with no microcarriers or external matrix required
• SCALE UP: Easily produce up to 1 x 10^9 hPSCs in as few as 2-3 weeks
• COST EFFECTIVE: Significant cost savings in both media and labor