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- 文献和实验
- 技术资料
- 应用范围:
Immunoprecipitation;Western Blotting
- 宿主:
0
- 库存:
大量
- 抗原来源:
0
- 是否单克隆:
0
- 规格:
10 mL
- Immunoprecipitation
- Western Blotting
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
详细描述见链接:http://www.millipore.com/catalogue/item/16-125
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文献和实验SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
, and then repeat Step 7. 8. Transfer the supernatant to a new nuclease-free 15 ml centrifuge tube containing one volume of 100% isopropanol. Do not transfer the protein pellet. 9. Gently mix the solution by inverting the tube 40-50 times. 10
10 mg/ml ethidium bromide To prepare 100 ml of a 0.7% agarose solution, measure 0.7 g agarose into a glass beaker or flask and add 100 ml 1X TBE or TAE. Microwave or stir on a hot plate until agarose is dissolved
lution of GST-Fusion Protein from Glutathione Agarose
lution of GST-Fusion Protein from Glutathione Agarose 1. Wash the GST-fusion protein glutathione-agarose complex once with 1% Triton X-100 in PBS. 2. Resuspend the complex in 1% Triton X-100 in PBS. 3. Prepare a small column
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