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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
18
- 保质期:
1年
- 供应商:
上海信裕生物科技有限公司
- 保存条件:
-20
- 规格:
100 μg /500 μg
| UvsX重组酶,来源于T4 噬菌体,是RecA/Rad51家族的同源体。RecA/Rad51重组酶家族在双链DNA断裂的无误修复和复制叉重新启动的过程中起到重要作用。T4 UvsX重组酶可与其他重组酶一起锚定在单链DNA上并激活其在其他双链DNA上寻找同源序列,以进一步完成链置换。该酶无核酸酶活性。 | ||||||||
组分 |
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应用 |
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1X UvsX Recombinase Buffer |
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| 20 mM Tris-acetate pH 7.8 100 mM Potassium acetate 10 mM Magnesium acetate 1 mM DTT | ||||||||
酶储存液 |
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| 50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 20% Glycerol, pH 7.5。 | ||||||||
储存 |
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| -20℃可保存2年,避免反复冻融 | ||||||||
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文献和实验Assay for Human Rad51-Mediated DNA Displacement Loop Formation
replication forks, and for several other aspects of chromosome metabolism and maintenance. The homologous recombination reaction is mediated by the Rad51 recombinase. In the presence of ATP, Rad51 polymerizes on single-stranded DNA (ssDNA) to form
Construction of Gene-Targeting Vectors by Recombineering
should be phenotypically normal unless they are bred to a Cre-expressing transgenic line. Depending on the nature of the promoter controlling Cre recombinase expression (ubiquitous or tissue-specific), deletion of the lox P-flanked ("floxed") region can occur
A Comprehensive Guide to Sleeping Beauty–Based Somatic Transposon Mutagenesis in the Mouse
recombinase (http://nagy.mshri.on.ca/cre_new/index.php) Conditional or non‐conditional predisposing transgene (optional
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