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- 文献和实验
- 技术资料
- 供应商：
上海研卉生物科技有限公司
- 英文名：
LDH-Cytotoxicity Assay Kit
- 保存条件：
-20℃
- 库存：
大量
- 规格：
400次分析
Kit Summary:
• Detection method- Absorbance (500 nm)
• Sample type- Cell culture (adherent and suspension)
• Species reactivity- Mammalian
• Applications- This assay provides a convenient and non-radioactive alternative to the [3H]-thymidine and the [51Cr] release assays The assay is based on measurement of activity of lactate dehydrogenase (LDH) which is a stable enzyme normally found in the cytosol of all cells but rapidly releases into the supernatant upon damage of plasma membrane.
Features & Benefits:
• Simple one-step procedure; takes less than 1 hour
• Fast, sensitive and convenient
Kit components:
• Catalyst, Lyophilized
• Dye Solution
Description:
Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. The LDH-Cytotoxicity Assay Kit provides a fast and simple method for quantitating cytotoxicity based on the measurement of activity of lactate dehydrogenase (LDH) released from damaged cells. Unlike many other cytoplasmic enzymes which exist in many cells either in low amount (e.g., alkaline and acid phosphatase) or unstable, LDH is a stable cytoplasmic enzyme present in all cells and rapidly released into the cell culture supernatant upon damage of the plasma membrane. LDH activity can be determined by a coupled enzymatic reaction: LDH oxidizes lactate to pyruvate which then reacts with tetrazolium salt INT to form formazan. The increase in the amount of formazan produced in culture supernatant directly correlates to the increase in the number of lysed cells. The formazan dye is water-soluble and can be detected by spectrophotometer at 500 nm. The LDH-cytotoxicity assay is sensitive, convenient, and precise, and is applicable to a variety of cytotoxicity studies. Assay takes ~0.5–1 hr.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.
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文献和实验1. 原理：LDH (乳酸脱氢酶） 是稳定的胞浆酶，存在所有的细胞中，当胞膜损伤时快速释放到细胞培养液中。 LDH 活性通过两个酶催化反应： LDH 氧化乳酸盐生成丙酮酸盐，然后丙酮酸盐和四唑盐 INT 反应生成甲（ formazan ）结晶。甲（ formazan ）结晶量在培养液中的增加，与裂解的细胞数增加直接相关。甲（ formazan ）结晶染料是水溶的，可以用分光光度计在 500nm 波长检测。通过检测细胞培养上清中
基因敲除模型：NLRP3−/−、GSDMD−/−、caspase-1/11−/−小鼠、RACK1ΔMΦ。 4、检测内容：LDH（乳酸脱氢酶）释放实验检测细胞毒性、ELISA 检测细胞因子分泌、WB 检测、免疫沉淀（IP）、pull down、质谱、EST12 晶体结构、共聚焦显微镜、小鼠结核感染模型等。 研究结果： 1、EST12（Rv1579c）诱导 GSDMD 介导的巨噬细胞焦亡 表达纯化 40 种 RD 区编码蛋白，通过 LDH 释放实验，发现 EST12（Rv1579c）具有明显的巨噬细胞毒性
大鼠 乳酸脱氢酶（LDH ） 酶联免疫分析 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定大鼠血清，血浆及相关液体样本中 乳酸脱氢酶（ LDH ） 的含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中 大鼠 乳酸脱氢酶（ LDH ） 水平。用纯化的 大鼠 乳酸脱氢酶（ LDH ） 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 乳酸脱氢酶（ LDH ） ，再与 HRP 标记
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