- ￥4329 - 8775
- Biorbyt
- orb639844
- 英国
- 2026年07月28日
- IHC-P
- Mouse
- Human
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- 文献和实验
- 技术资料
- 抗体名：
OGG1 Antibody / 8-Oxoguanine DNA Glycosylase 抗体
- 抗体英文名：
OGG1 Antibody / 8-Oxoguanine DNA Glycosylase
- 应用范围：
IHC-P
- 宿主：
Mouse
- 适应物种：
Human
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb639844
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
Mouse IgG2c, kappa
- 免疫原：
详询
- 规格：
20 ug/100 ug
| 规格： | 20 ug | 产品价格： | ￥4329.0 |
|---|---|---|---|
| 规格： | 100 ug | 产品价格： | ￥8775.0 |
产品子类型: Primary Antibody
描述: 8-oxoguanine (8-oxoG), an oxidized form of guanine, is produced by reactive oxygen species in both DNA and nucleotide pools during normal aging. Accumulation of 8-oxoG increases the occurrence of A:T to C:G or G:C to T:A transversionmutations, because 8-oxoG forms a stable basepair with adenine as well as with cytosine. OGG1 (for 8-oxoG DNA glycosylase), also designated MMH, is a DNA repair enzyme that corrects these mutations. Inactivation of the OGG1 gene leads to a mutator phenotype, characterized by the increase in G:C to T:A transversions. The OGG1 gene encodes eight isoforms (OGG1A-C, OGG2A-E) which result from alternative splicing of a single messenger RNA. The OGG1A splice variant is the most prevalent form and localizes to the nucleus, whereas the OGG2A splice variant is targeted to the mitochondria. Guanine is the main target for reactive oxygen species in DNA, and 8-oxoguanine is the most frequent base lesion. Therefore, formation of 8-oxoguanine is an important biomarker of oxidative damage to DNA. It is primarily repaired by the DNA glycosylase OGG1. Furthermore, defects in OGG1 may be a cause of renal cell carcinoma.
克隆号: CPTC-OGG1-1
应用稀释比例: Immunohistochemistry (FFPE): 1-2ug/ml
应用说明: Optimal dilution of the OGG1 antibody should be determined by the researcher.
免疫原: A recombinant full-length human protein was used as the immunogen for this OGG1 antibody.
防腐剂: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
纯化方法: Protein G affinity chromatography
研究领域: Epigenetics & Chromatin
UniProt ID: O15527
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验-treatment of samples with DNase I or MutM, the latter excising 8-oxoG opposite C in DNA. We then analyzed 8-oxoG dynamics in mitochondrial DNA of the wild-type and 8-oxoG DNA glycosylase (OGG1)-deficient mouse cells after exposure to hydrogen peroxide
in such cell death. By introducing human cDNA encoding a nuclear form of 8-oxoG DNA glycosylase (hOGG1-1a) into immortalized mouse embryo fibroblasts lacking Ogg1 gene, we established a cell line which selectively accumulates 8-oxoguanine in mitochondrial DNA
和化学切割 ( 1 ) 大肠杆菌尿嘧啶-DNA-糖基化酶（UDG) ( NEB 或 PeqlabbiotechnologieGmbH 公司）。 ( 2 ) 99.9% 的哌啶（Sigma 公司）。 2.4 尿素变性聚丙烯酰胺凝胶电泳 ( 1 ) 尿素（纯度 > 99.5%)。 ( 2 ) 30% 聚丙烯酰胺，0.8% 甲叉双丙烯酰胺（37.5 : 1 ) 储液（Carl Roth GmbH 公司）。 ( 3 ) 10 X TBE 缓冲液（见 10.2.2
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