- 询价
- 13-110
- 2025年07月14日
- Kinase Assay
- 0
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- 询价记录
- 文献和实验
- 技术资料
- 应用范围：
Kinase Assay
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- 库存：
大量
- 是否单克隆：
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- 规格：
5 mg
Product Images
Immunoblot analysis: 800ng MBP incubated without (lane 1) or with (lane 2) MAPK 2 and probed with anti phospho MBP (1μg/ml)
- MBP
- MGC99675
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文献和实验Factor Xa Cleavage of MBP-Fusion protein
following ON treatment at RT with 10-30 ug Factor Xa from New England BioLabs per mg fusion protein. For some applications factor Xa (MW 43kDa consisting of two subunits of 27 and 16kDa) must be subsequently removed from the sample by chromatography
Purification of MBP (maLTose-binding proteins) Fused Proteins
. Resuspend pellet in 100 ml buffer for analysis on SDS-PAGE. Elute "unbound" proteins from amylose resin by washing resin with 10 column volumes of 20 mM Tris pH7.4, 200 mM NaCl, 10mM b ME, 1 mM EDTA. Elute bound MBP fusion protein
Protein Expression and Purification of MBP-T antigen (131-259)
Comments: T antigen (131-259)is an MBP-Hisx6-fusion protein constructed by Laura Mizoue (refer to the wisdom page for details on the construct). i.Pick a single colony from freshly transformed cells (BL21 (DE3)is the expression host)into 2 ml of LB
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