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文献和实验In Vitro PCNA Modification Assays
Modification of the replicative sliding clamp, PCNA, by monoubiquitin, polyubiquitin, and SUMO contributes to the processing of DNA damage during replication. In order to investigate the properties of the relevant conjugation enzymes
Detection of PCNA Modifications in Saccharomyces cerevisiae
PCNA modifications by members of the ubiquitin family are associated with a range of different transactions during replication of damaged and undamaged DNA. This chapter describes detailed protocols for the detection and isolation
Flow Cytometric Detection of Proliferation-Associated Antigens, PCNA and Ki-67
The rapid evolution of monoclonal antibody (MAb) technology has resulted in an ever-growing repertoire of proteins becoming accessible for study using immunohistochemical techniques. Nuclear antigens represent a class of proteins
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