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- Cedarlane
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- 2025年07月12日
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文献和实验Clone Genes From a Phage Library
may be amplified if you wish, but this should not be necessary. It was made by cutting the genomic DNA with Tsp509 I at several different concentrations and size-selecting pieces from 4 to 8-9 kb from each digestion to ligate into the Lambda ZapII vector (at Eco RI
Clone Genes From a Phage Library
which is the lambda receptor) overnight at 30 degrees, spin down in a 50 ml Falcon tube, and resuspend to half volume in 10 mM MgSO4. These bacteria will be good to use for phage plating for several weeks.Use 50 microliters per plate. Filters: I like simple uncharged
Clone Genes From a Phage Librar
+ 0.2 percent maltose (to express the mal permease which is the lambda receptor) overnight at 30 degrees, spin down in a 50 ml Falcon tube, and resuspend to half volume in 10 mM MgSO4. These bacteria will be good to use for phage plating
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