- 询价
- 07-507
- 2025年07月08日
- Western Blotting
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Western Blotting
- 宿主：
Rabbit
- 抗原来源：
0
- 库存：
大量
- 适应物种：
Human;Mouse;Rat
- 是否单克隆：
2
- 规格：
200 μL
Product Images
WB: Lysates from untreated (Lanes 1,3) or sorbitol-treated (Lanes 2,4) HeLa cells were probed with either anti-phospho-BMK1/Erk5 (Thr218, Tyr220) (1:...
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | Rabbit | Affinity Purified | Polyclonal Antibody |
- Human
- Mouse
- Rat
- MAPK7
- ERK5
- BMK1
- ERK-5
- ERK4
- PRKM7
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
KLH-conjugated, synthetic peptide corresponding to amino acids 213-224 of human BMK1/Erk5 详细描述见链接：http://www.millipore.com/catalogue/item/07-507
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文献和实验v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
Assays (See Note 9) 1. Pre-incubate anti-GSK3β (5 μg) monoclonal antibody withprotein G-Sepharose (400 μL of a 50% slurry) in kinasebuffer at 4°C for 1 h to conjugate the antibody to the proteinG-Sepharose beads. 2. Centrifuge the beads at 6,000× g
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
, which may be used to specifically detect Ser, Thr, Ser/Thr, or Tyr residues phosphorylated by different protein kinase families. These antibodies are commonly used in immunoprecipitation and/or immunoblotting protocols to determine kinase?induced substrate
secondary antibody were purchased from GIBCO-BRL. Phosphospecific rabbit polyclonal antibodies against 180Thr and 182Tyr dual-phosphorylated p38, against total p38 (phosphorylation state independent), against 204Tyr-phosphorylated ERK1/2 and against total
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