Neutrophil (mouse) Isolation K

中性粒细胞是短命的炎症细胞，非常擅长吞噬和破坏细菌，是针对许多致病生物的先天免疫反应的第一道防线。它们产生大量防御性抗菌蛋白和肽，包括溶菌酶、髓过氧化物酶、中性粒细胞弹性蛋白酶、组织蛋白酶以及α和β防御素。中性粒细胞还形成细胞外陷阱，这是一种由DNA、组蛋白和各种抗菌蛋白组成的网状结构，从中性粒细胞中挤出以诱捕和杀死细胞外病原体。在小鼠中，中性粒细胞占循环白细胞的约10%，因此与人类不同，通常不可能从一只小鼠的外周血中分离出哪怕一百万个中性粒细胞。Cayman的中性粒细胞（小鼠）分离试剂盒提供了一种通过腹膜灌洗分离小鼠中性粒细胞的简化方法。该试剂盒包含在多达五只小鼠的腹腔中诱导无菌炎症反应所需的所有试剂、将中性粒细胞与其他腹膜白细胞分离所需的密度分离介质，以及通过流式细胞术评估分离过程成功所需的荧光染料标记抗体。Neutrophils are short-lived inflammatory cells that are highly adept at ingesting and destroying bacteria as part of the first line of defense in the innate immune response to many pathogenic organisms. They produce an extensive arsenal of defensive antimicrobial proteins and peptides including lysozyme, myeloperoxidase, neutrophil elastase, cathepsins, and the alpha- and beta-defensins. Neutrophils also form extracellular traps, web-like structures composed of DNA, histones, and various antimicrobial proteins that are extruded from the neutrophil in order to ensnare and kill extracellular pathogens. In mice, neutrophils comprise ~10% of circulating white blood cells, so unlike humans, it is usually not possible to isolate even one million neutrophils from a single mouse from peripheral blood. Cayman's Neutrophil (mouse) Isolation Kit provides a streamlined method for isolating mouse neutrophils by peritoneal lavage. Included in this kit are all the reagents necessary for inducing a sterile inflammatory response in the peritoneal cavities of up to five mice, the density separation medium required to separate the neutrophils from other peritoneal leukocytes, and the fluorochrome-labeled antibodies required to assess the success of the isolation procedure by flow cytometry.分子式分子量