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文献和实验:6:0.8 of chloroform-methanol-water. ---> NOTE: It is best not to extract at this point because gangliosides could potentially partition into the aqueous phase and be lost. ---> This mixture can be stored in the freezer for short periods of time. 9) Prepare
:6:0.8 of chloroform-methanol-water. ---> NOTE: It is best not to extract at this point because gangliosides could potentially partition into the aqueous phase and be lost. ---> This mixture can be stored in the freezer for short periods of time. 9) Prepare
Glycosphingolipid analysis Contributor: Suprya Jayadev Date: Nov. 10, 1993 1) Incubate cells with 1 µCi/ml of 3H-galactose for 72 hours. ---> If treatment is for an extended period of time: treat in serum free
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