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- 27179-1 mg
- 2026年04月15日
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文献和实验7, where both primers have a similar melting point with primers for locus 6 (58° C). They are not as well amplified in 1x buffer, but respond well to increase in the salt concentration. In this case, the explanation may be that the entire product 7 has a lower GC
-deletions or to cell exposure to folding or degradation inhibitors.
Addition of 3-A Overhangs (A-Tailing) to PCR Product
and use it immediately for ligation reaction with a TA cloning vector such as the TOPO TA cloning vector from Invitrogen. Alternatively, the PCR product can be purified before used in ligation reaction as follows. Extract reaction immediately
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