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文献和实验2 Dimensional Gel Electrophoretic Analysis for Chicken Egg
Overview This protocol is a detail description of the procedure in performing 2D gel electrophoresis for illustrating the protein profile of the whole chicken egg. A similar protocol was presented by C.Desert, C.Gue''in-Dubiard, F.Nau, G
Single-Cell Method for Estimation of Protein Tyrosine Kinases in the Zebrafish Egg
from a single egg and then solubilize this crude fraction in a nonionic detergent and do an autophos-phosphorylation assay using high-specific-activity [γ-32 P]ATP. When the reaction products are resolved on sodium dodecyl sulfate (SDS) gels and treated
of functions, including microtubule network organization and cargo transportation along microtubules. Here, we describe the use of Taxol-stabilized microtubules for purification of MAPs, motors, and their complexes from Xenopus egg extracts. Isolated proteins
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