Isoginkgetin

一种双黄酮前mRNA剪接抑制剂；在33μM时降低mRNA依赖性荧光素酶报告基因的活性，将总RNA提取物从以mRNA为主转变为以前mRNA为主，并在HEK293细胞中阻止细胞生长；在10μM时抑制Con A和LPS诱导的小鼠淋巴细胞增殖；在10μM时抑制PMA和A23187诱导的大鼠腹腔巨噬细胞中花生四烯酸的释放；在5-20μM浓度下，剂量依赖性地减少HT1080纤维肉瘤细胞的MMP-9活性、表达和mRNA水平，并减少细胞侵袭。A biflavonoid inhibitor of pre-mRNA splicing; reduces the activity of an mRNA-dependent luciferase reporter, shifts the total RNA extract from predominantly mRNA to pre-mRNA, and arrests cell growth in HEK293 cells at 33 μM; suppresses Con A- and LPS-induced mouse lymphocyte proliferation at 10 μM; suppresses PMA- and A23187-induced arachidonic acid release in rat peritoneal macrophages at 10 μM; dose-dependently reduces MMP-9 activity, expression, and mRNA levels in and decreases cell invasion by HT1080 fibrosarcoma cells from 5-20 μM.