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文献和实验Peptide Pull-Down (PPD) Assay for Identification and Characterization of Histone PTM Effectors
to any peptide-conjugated beads being tested Other buffers besides HEPES can be used in this assay: PBS or Tris buffers at various salt concentrations have not had obvious detrimental effects in our PPDs The following is written for a single
28 , 730–738, 2007). This chapter presents a very efficient way to detect potential phosphorylation sites in protein families using overlapping peptides covering the complete primary structures (peptide scans) immobilized on glass slides.
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
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