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文献和实验The presence of a redundant lipolytic system of bile salt-activated lipase (BAL) and pancreatic lipase results in the concerted action of these lipolytic enzymes during intestinal dietary fat digestion processes (1 ). Because of its wide
1. Cut 0.5 cm of tail and mark mouse ears or toes 2. Incubate at 55°C O/N in: 500 l Tail Extraction Buffer 10 l Proteinase K (10 mg/ml) 12.5 l 20% SDS 3. Add 150 l 5 M NaCl
Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
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