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- 2026年04月15日
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文献和实验Interaction Trap/Two‐Hybrid System to Identify Interacting Proteins
method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
) generating a so called GFP-Trap. A direct comparison of the GFP-Trap with conventional antibodies for immunoprecipitation of GFP from crude cell lysates reveal that the GFP-Trap allows a very fast (~ 5 � 30 min) depletion of GFP from tested samples
interest in developing techniques to characterize “secretomes.” Here, we describe the use of the yeast secretion trap (YST) functional screen to isolate and identify secreted proteins that are accumulated and detected in the extracellular matrix of eukaryotes
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