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文献和实验Interaction Trap/Two‐Hybrid System to Identify Interacting Proteins
method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
) generating a so called GFP-Trap. A direct comparison of the GFP-Trap with conventional antibodies for immunoprecipitation of GFP from crude cell lysates reveal that the GFP-Trap allows a very fast (~ 5 � 30 min) depletion of GFP from tested samples
Application of Amide Proton Exchange Mass Spectrometry for the Study of Protein‐Protein Interactions
describes amide proton exchange experiments that probe for changes in solvent accessibility at protein?protein interfaces. The simplest version of the protocol, termed the ?on?exchange? experiment, detects protein?protein interfaces by taking advantage
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