- ￥2506
- Cayman
- 17097-50 mg
- 2025年11月08日
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文献和实验Measurement of Reduced Glutathione Using High-Pressure Liquid Chromatography
-glutamyl transpeptidase. In order to obtain a true measurement of the amount of reduced glutathione (GSH) in living cells we have utilized the method of Cotgreave and Moldeus ( 1 ) in which GSH is derivatized using monobromobimane (MBBr), which can freely
Assessment of Glutathione Homeostasis
, GSH levels are measured after being derivatized using the fluorescent thiol reactive compound monobromobimane (MBB) and are detected by high-performance liquid chromatography (HPLC). The third assay allows the assessment of GCL activity, also by HPLC.
-4-sulfonate (15 ,16 ), methyl 4-(6-methoxynaphthalene-2-yl)-4-oxo-2-butenoate (17 ), N-(1-pyrenyl)maleimide (18 ), monobromobimane (19 –23 ), o -phthaldialdehyde (24 ,25 ), 9-fluorenylmethyloxycarbonyl chloride (26 ), and 2-chloro-1-methylpyridinium
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