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- 2025年07月16日
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文献和实验Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
Materials 1.Drop frozen 2x cycled tubulin in a polymerization-promoting buffer.Stored at -80℃ 2.GTP,100 mM stock,titrate to pH ~7.Need 2 ml. 3.Waterbath,set at 37℃ 4.SS34 rotor,5 ml and 15 ml tubes and adaptors.Bring the rotor,adaptors,and tubes
LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
,collect 10 drop fractions. 13.Collect fluorescent fractions in the void volume,measure volume in a volumetric conical tube. 14.Polymerize actin by adding KCl to 100 mM and MgCl2 to 2 mM.Incubate for 30-60 min at room temperature. 15.Centrifuge in a 50Ti
In an effort to produce renm mhibttory peptides, the preparation of (2 S ,4 S ,5 S )-5-( t -Butoxycarbonylamino)-4-( t -butyldimethylsilyloxy)-2-isopropyl-7-methyl octanotc acid, 15 , was carried out ( 1 ). This pseudo-dipeptide was used
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