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文献和实验RNA Trans-Splicing for Genodermatoses
Spliceosome-mediated RNA trans -splicing (SMaRT) is a tool that facilitates the recombination of two distinct pre-mRNA molecules. Its application for gene therapeutic purposes has been hindered by laborious procedures to identify gene
In Vitro Trans-Translation Assays
Trans -translation is a bacterial quality control system in protein synthesis facilitated by transfer-messenger RNA (tmRNA). Here, we describe the in vitro system using purified factors to evaluate the two steps of trans -translation
Trans-Splicing Reactions by Ribozymes
Trans -cleaving ribozymes can be targeted to cut specific RNAs in vitro, which has led to much interest in their potential to destroy specific messages inside cells. Here, we will describe a different reaction catalyzed by ribozymes
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