- ￥1260
- Cayman
- 16225-500 mg
- 2025年07月12日
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文献和实验Ahr knockout genotyping by PCR
parameters: 95 C/10min -> [95 C/1min -> 55 C/1min -> 72 C/10sec]x 35 -> 72 C/15min Ahr KO using Promega Taq Master Mix: 5 ul Promega 10 x buffer without MgCl2 4 ul 25 mMgC12 1 ul of each dNTP 1.5 ul Ol 72 (5 uM) 1.5 ul Ol 111 (5 uM
Ahr transgenic genotyping by PCR
. Run 10 ul from each PCR reaction out on a 1% agarose gel. Master Mix: 5 ul 10xPCR buffer (with MgCl2, 1.5 mM final) 4 ul dNTP mix (2.5 mM each) 1 ul OL638 (20 uM) 1 ul OL639 (20 uM) 0.5 ul Taq 34.5 ul distilled, deionized
ARNT transgenic genotyping by PCR
Bradfield Lab, McArdle Laboratory for Cancer Research, University of Wisconsin-Madison Medical School http://mcardle.oncology.wisc.edu/bradfield/ARNT-transgenic-genotyping.html Amplify a 700bp fragment using OL637 and OL639. Run 10
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