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文献和实验Polyclonal and Monoclonal Antibodies
Polyclonal Antibody Immunization of Mice: Injections should be made at intervals of at least two weeks.. We have been using either of the two following adjuvants with success: Freund adjuvant and MPL+TDM adjuvant ( RIBI ImmunoChem
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
Polyclonal Antibody Production 简单快捷制备多抗方法,可用于western、ELISA和免疫/b
the hair away from the injection site and sterilize with a squirt of ethanol. Pinch the skin and pull up slightly to pull the skin away from the muscle, insert the needle 1-2 cm at a 15 degree angle so as to not hit muscle, and inject 250 µL
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