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文献和实验, or other biologically active molecules. In this chapter, we present a technique, which allows identification of specific DNA sequences by fluorescence in situ hybridization (FISH) and simultaneously analyses the incorporation of a thymidine analogue, 5-bromo
Multiparameter Cell Cycle Analysis
, which has been well-published and reviewed. Next most common is analysis of 5-bromo-2-deoxyuridine (BrdU) incorporation, detected by specific antibodies – also well-published and reviewed. A new measurement approach to S phase labeling utilizes 5′-ethynyl-2′-deoxyuridine (EdU
Proliferation Assays (BrdU and EdU) on Skeletal Tissue Sections
with specific steps of the cell cycle, but the gold standard is to quantify the percentage of DNA-synthesizing cells. The thymidine analog 5-bromo-2′-deoxyuridine (BrdU) has been widely used in the last decades for this purpose, with the inconvenience
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