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文献和实验. Chen, C.D., Oh, S.Y., Hinman, J.D., and Abraham, C.R. 2006. Visualization of APP dimerization and APP‐Notch2 heterodimerization in living cells using bimolecular fluorescence complementation. J. Neurochem. 97
syringe, NORM-JECT (1 ml Ins/TBC; Henke-Sass & Wolf) Critical This should be free of latex and silicon oils. Refrigerated table-top centrifuge (Eppendorf, cat. no. 5415 R) Reaction tubes, polycarbonate, 0.2 ml (Beckman, cat
A Simple and Reliable Assay of Proteoglycan Synthesis by Cultured Chondrocytes
A simple and reliable method to measure proteoglycan synthesis by chondrocytes in culture is described. Confluent chondrocytes in 24-well plates are labeled for 24–72 h with 35 SO4 2− in the presence of stimulating agents. At the end
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