IκBα (Phospho-Ser32/36) Monocl

抗原：含有与人类IκBα氨基酸32和36相对应的磷酸化丝氨酸残基的合成肽；宿主：小鼠，克隆39A1413；同种型：IgG1κ；交叉反应：(+)牛、犬、人、小鼠、猪和大鼠IκBα；应用：WB和IP；外部刺激（如肿瘤坏死因子或其他细胞因子）会启动信号转导级联，导致IκB-激酶复合物的激活，该复合物特异性地磷酸化丝氨酸32和丝氨酸36上的IκBα。这些位点的磷酸化导致IκBα泛素化，随后被26S蛋白酶体降解。IκBα降解导致NF-κB二聚体的核定位信号暴露，随后转移到细胞核并激活靶基因。Antigen: synthetic peptide containing phosphorylated serine residues corresponding to human IκBα amino acids 32 and 36; Host: mouse, clone 39A1413; Isotype: IgG1κ; Cross Reactivity: (+) bovine, canine, human, mouse, porcine, and rat IκBα; Application(s): WB and IP; External stimuli such as tumor necrosis factor or other cytokines initiates a signal transduction cascade that leads to activation of the IκB-kinase complex that specifically phosphorylates IκBα on serine32 and serine36. Phosphorylation of these sites leads to ubiquitination of IκBα and subsequent degradation by the 26S proteasome. Degradation of IκBα results in unmasking of the nuclear localization signal of NF-κB dimers, which subsequently translocate to the nucleus and activate target genes.