Xanthine Oxidase Fluorometric

XO催化次黄嘌呤羟基化为黄嘌呤，然后进一步催化黄嘌呤氧化为尿酸。当氧化NADH时，XO会产生超氧化物，这是一种强大的ROS。XO主要存在于肝脏和肠道中。然而，疾病会导致循环中XO的水平急剧增加。Cayman的XO检测提供了一种简单而准确的方法来量化黄嘌呤氧化酶活性。该检测基于多步酶促反应，其最终产物雷索鲁芬是一种高荧光化合物，可以使用520-550nm之间的激发波长和585-595nm之间的发射波长轻松分析。XO catalyzes the hydroxylation of hypoxanthine to xanthine and then further catalyzes the oxidation of xanthine to uric acid. When oxidizing NADH, XO generates superoxide, a powerful ROS. XO is found predominantly in the liver and intestine. However, levels of circulating XO can increase dramatically with disease. Cayman's XO Assay provides a simple and accurate method for quantifying xanthine oxidase activity. The assay is based on a multistep enzymatic reaction whose end product resorufin, a highly fluorescent compound, can be easily analyzed using an excitation wavelength between 520-550 nm and an emission wavelength between 585-595 nm.