Glycerol Colorimetric Assay Ki

甘油是甘油三酯的骨干，也是能量代谢中的重要中间体，参与氧化和合成过程。甘油和游离脂肪酸的循环水平被认为反映了脂肪分解，因此是评估各种情况的有用参数。在测量参考材料和甘油浓度升高的患者血清中的甘油三酯时，测量甘油也有助于校正甘油干扰。Cayman的甘油测定法提供了一种简单、可重复且灵敏的工具，用于测量血浆和血清中的甘油。该测定法采用偶联酶反应系统，可产生吸光度最大为540nm的亮紫色产物。Glycerol is the backbone of triglycerides and an important intermediate in energy metabolism being involved in both oxidative and synthetic processes. The circulating levels of glycerol and free fatty acids are considered to reflect lipolysis and are therefore useful parameters to evaluate in various conditions. The measurement of glycerol is also useful for correction of glycerol interference when measuring triglycerides in reference materials and in serum from patients with elevated glycerol concentrations. Cayman's Glycerol Assay provides a simple, reproducible, and sensitive tool for measurement of glycerol in plasma and serum. The assay employs a coupled enzymatic reaction system that yields a brilliant purple product with an absorbance maximum at 540 nm.