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文献和实验Analysis of Protein Prenylation and S-Acylation Using Gas ChromatographyCoupled Mass Spectrometry
attachment of either farnesyl (15 carbons) or geranylgeranyl (20 carbons) isoprenoids to conserved cysteine residues at or near the C-terminus of proteins. S -acylation, frequently referred to as palmitoylation, involves the attachment of acyl fatty acids
Genetic Modification in Bacillus subtilis for Production of C30 Carotenoids
pathway is dehydrosqualene synthase CrtM. CrtM converts farnesyl pyrophosphate to dehydrosqualene. Dehydrosqualene desaturase CrtN then converts dehydrosqualene to the yellow C30 carotenoid, 4,4′-diaponeurosporene. This chapter describes a method
Metabolic Labeling of Prenyl and Carboxyl‐Methyl Groups
protocols for prenylation and carboxy?methylation of proteins in cultured cells. These modifications often accompany fatty acid acylation. Cultured cells can be labeled biosynthetically using radiolabeled mevalonate, a precursor, to label intermediates
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